Your search for " Upstate " resulted in 2 protocols
Immunoprecipitation/Kinase Assay Author: Source:Upstate Abstract: This protocol is applicable only to kinases whose activity is not altered by cell lysis or immunoprecipitation procedures and that do not require soluble cofactors for activity.
General Immunoprecipitation Phosphatase Assay Protocol Author: Source:Upstate Abstract: To assess for specific phosphatase activity. the phosphate levels detected with protein A or G-agarose beads incubated with cell lysates in the absence of antibody is used as a background. Buffer components must be checked for any free phosphate contamination.
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