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Restriction enzyme digestion of DNA: basic method Author: Matt Lewis Source: Department of Pathology. University of Liverpool Abstract: 0.5µl of enzyme is plenty for a miniprep digestion. Do not use more enzyme than 10% of the final reaction volume (ie. not more than 1µl in this case). This is because the enzyme storage buffer contains antifreeze (glycerol) to allow it to survive at –20°C. The glycerol will inhibit the digestion if present in sufficient quantities.
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