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Methods for the Measurement of a Bacterial Enzyme Activity in Cell Lysates and Extracts Author: Brendan Burns. George Mendz and Stuart Hazell Source: School of Microbiology and Immunology. The University of New South Wales Abstract: Studies of bacterial enzyme activities provide fundamental information relevant to microbial physiology. and to a more complete understanding of cell metabolism. bacterial evolution. and to the interactions that occurs between bacterium and host. Characterizing enzyme activities helps to elucidate regulatory mechanisms and pathways in an organism. and to identify proteins essential for cell survival.