Your search for " Fermentas International Inc " resulted in 8 protocols
Protocol for PCR with Hot Start Taq DNA Polymerase Author: Source:Fermentas International Inc Abstract: Optimal amounts of template DNA in the 50μl reaction volume are in the 0.01-1ng range for both plasmid and phage DNA. and in the 0.1-1μg
range for genomic DNA. Higher amounts of template increase the risk of generation of non-specific PCR products. Lower amounts of template
reduce the accuracy of the amplificati
Protocol for PCR with Taq DNA Polymerase Author: Source:Fermentas International Inc Abstract: PCR allows the production of more than 10 million copies of a target DNA sequence from only a few molecules. The sensitivity of this technique means that the sample should not be contaminated with any other DNA or previously amplified products (amplicons) that may reside in the laboratory environment.
Protocol for PCR with Pfu DNA Polymerase Author: Source:Fermentas International Inc Abstract: DNA sample preparation. reaction mixture assemblage and the PCR process. in addition to the subsequent reaction product analysis. should be performed in separate areas.
A Laminar Flow Cabinet equipped with a UV lamp is recommended for preparing the reaction mixture.
Fresh gloves should be worn for each PCR step.
The use of dedicated vessels and positive displacement pipettes or tips with aerosol filters for both DNA sample and reaction mixture preparation. is strongly recommended.
M-MuLV Reverse Transcriptase Author: Source:Fermentas International Inc Abstract: The M-MuLV Reverse Transcriptase (RT) is an RNA- and DNA-dependent DNA polymerase. It can use either RNA or DNA to prime DNA synthesis. The enzyme possesses a ribonuclease H activity specific to RNA in RNA-DNA hybrids
AMV Reverse Transcriptase Author: Source:Fermentas International Inc Abstract: AMV Reverse Transcriptase (RT) is a recombinant Avian Myeloblastosis Virus reverse transcriptase expressed in E.coli. AMV RT is a heterodimer composed of nonidentical subunits alfa and beta. It possesses multiple enzymatic activities including RNA- and DNA-directed DNA polymerase. DNA-RNA unwinding activity. a sequence-specific Mn2+-dependent endonuclease and ribonuclease H.
DNA Polymerase I. E.coli Author: Source:Fermentas International Inc Abstract: The DNA Polymerase I. a template-dependent DNA polymerase. catalyzes 5’=>3’ synthesis of DNA. The enzyme also exhibits 3’=>5’ exonuclease (proofreading) activity. 5’=>3’ exonuclease activity and ribonuclease H activity.
Terminal Deoxynucleotidyl Transferase Author: Source:Fermentas International Inc Abstract: The Terminal Deoxynucleotidyl Transferase (TdT). a template-independent DNA polymerase. catalyzes the repetitive addition of deoxyribonucleotides to the 3’-OH of oligodeoxyribonucleotide and single-stranded. and double-stranded DNA (1). The TdT requires an oligonucleotide of at least three nucleotides to serve as a primer.
Bacterial Alkaline Phosphatase Author: Source:Fermentas International Inc Abstract: The Bacterial Alkaline Phosphatase (BAP) catalyzes the release of 5'- and 3'-phosphate groups from DNA. RNA and nucleotides. Also. this enzyme can remove phosphate groups from proteins.
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