Your search for " Biologicalprocedures " resulted in 5 protocols
Measuring Solution Viscosity and its Effect on Enzyme Activity Author: Salvador Uribe and José G. Sampedro Source:Biologicalprocedures Abstract: In proteins. some processes require conformational changes
involving structural domain diffusion. Among these processes
are protein folding. unfolding and enzyme catalysis. During
catalysis some enzymes undergo large conformational changes
as they progress through the catalytic cycle.
An in vitro assay to study the recruitment and substrate specificity of chromatin modifying enzymes Author: Michiel Vermeulen and Hendrik G. Stunnenberg Source:Biologicalprocedures Abstract: Post-translational modifications of core histones play an important role in regulating fundamental biological processes such as DNA
repair. transcription and replication.This paper describes a novel assay that allows sequential targeting of distinct histone
modifying enzymes to immobilized nucleosomal templates using recombinant chimeric targeting molecules.
Use of Site-Specifically Tethered Chemical Nucleases to Study Macromolecular Reactions Author: Srabani Mukherjee and Rui Sousa Source:Biologicalprocedures Abstract: During a complex macromolecular reaction multiple changes in
molecular conformation and interactions with ligands may
occur. X-ray crystallography may provide only a limited set of
snapshots of these changes.