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Your search for " Biologicalprocedures " resulted in 5 protocols
Measuring Solution Viscosity and its Effect on Enzyme Activity
Author: Salvador Uribe and José G. Sampedro
Source: Biologicalprocedures
Abstract: In proteins. some processes require conformational changes involving structural domain diffusion. Among these processes are protein folding. unfolding and enzyme catalysis. During catalysis some enzymes undergo large conformational changes as they progress through the catalytic cycle.

An in vitro assay to study the recruitment and substrate specificity of chromatin modifying enzymes
Author: Michiel Vermeulen and Hendrik G. Stunnenberg
Source: Biologicalprocedures
Abstract: Post-translational modifications of core histones play an important role in regulating fundamental biological processes such as DNA repair. transcription and replication.This paper describes a novel assay that allows sequential targeting of distinct histone modifying enzymes to immobilized nucleosomal templates using recombinant chimeric targeting molecules.

A fluorescence microscopy method for quantifying levels of prostaglandin endoperoxide H synthase-1 and CD-41 in MEG-01 cells
Author: Douglas J Franks. Cameron Mroske and Odette Laneuville
Source: Biologicalprocedures
Abstract: In platelets. PGHS-1-dependant formation of thromboxane A2 is an important modulator of platelet function and a target for pharmacological inhibition of platelet function by aspirin.

Use of Site-Specifically Tethered Chemical Nucleases to Study Macromolecular Reactions
Author: Srabani Mukherjee and Rui Sousa
Source: Biologicalprocedures
Abstract: During a complex macromolecular reaction multiple changes in molecular conformation and interactions with ligands may occur. X-ray crystallography may provide only a limited set of snapshots of these changes.

Investigation of Neuronal Cell Type-Specific Gene Expression of Ca2+/Calmodulin-dependent Protein Kinase II.
Author: Kazuko Mima. Hitomi Donai and Takashi Yamauchi
Source: Biologicalprocedures
Abstract: The promoter activity of the rat Ca2+/calmodulin-dependent protein kinase II gene was analyzed using the luciferase reporter gene in neuronal and non-neuronal cell lines.

 
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