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General Protocol for Sandwich-style Enzyme-Linked Immunosorbent Assay (ELISA) Author: Source:Affinity Biologicals Abstract: Analytes in plasma and other fluids can be measured by capturing onto a microtitre plate coated with a capture antibody. After washing the plate to remove unbound proteins the captured analyte is detected by incubating with another antibody containing a reporter molecule. in this case the enzyme horseradish peroxidase.