ELISPOT (Enzyme Linked Immunospot)protocols Author: Source:Abcam Abstract: Cells are grown in 96 well plates with antibody-coated PVDF or nitrocellulose membranes to the wells. First. the membrane is coated with primary antibody.
Direct ELISA protocol using fluorescent substrate Author: Source:Abcam Abstract: This technique can quantify a particular antibody by immobilising the antigen to a solid support and then applying the solution containing the antibody in question. Unbound antigen is washed off and the bound antibody visualised via an anti-immunoglobulin antibody conjugated to a suitable label.
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