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| PCR has become such a broadly applied technique that Taq polymerases now exist which can fulfill almost any need or niche imaginable. Standard Taq has an error rate that is reported to be anywhere from 1 in 10,000 to 1 in 100,000. This is more than adequate for general screening applications, but if sequence accuracy is critical for your work, you will want to choose a high-fidelity Taq. Many of these enzymes offer accuracies several fold greater than standard Taq. In addition, there are now modified Taq enzymes which can effectively perform "long PCR"; some are able to amplify up to 48 kb of DNA. Once you've chosen the Taq with the activity suited to your application, you can select from packaging options which will do everything from reduce the time required for your reaction set-up (master mixes) or your post-reaction analysis (e.g. REDTaqT which is packaged with loading dye) to improving your results (hot start enzymes). Before you make your final decision, remember that the more options you choose, the higher the price you're apt to pay. So if you just need the basics, choose a basic Taq - or perhaps a Taq-comparable enzyme that comes with all the standard activity, but none of the licensing fees. One thing's for sure, whatever your amplification needs, you'll be able to find the Taq to fulfill them by browsing through the links below |
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Tfi DNA Polymerase
Invitrogen
Tfi DNA Polymerase is a new non-Taq based, PCR enzyme derived from Thermus filiformis for use in PCR applications without any licensing fees. Like Taq, Tfi has 5'3' exonuclease and 5'3' polymerase activity. PCR performance is comparable to Taq DNA polymerase in yield, specificity, fidelity, and robustness.Tfi can be substituted for Taq DNA Polymerase in virtually any application and is ideal for genomic, cDNA, and plasmid targets up to 4 kb in length. In addition, Tfi is free of licensing restrictions that accompany Taq polymerase. |
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Taq Polymerase Products
Sigma-Aldrich
Sigma-Aldrich provides its customers with the broadest range of products and capabilities available today, making it the leading choice in nucleic acid amplification. From the direct load capability of Sigma’s patented line of RED enzymes to the DNA repair enzyme, Restorase® or low-DNA MTP™ Taq, Sigma is continually striving to provide you with high quality products at a great value. Sigma's JumpStart™ Taq polymerase is an antibody-inactivated hot start enzyme designed to minimize non-specific amplification while increasing target yield. |
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Taq Polymerase Reagents
Upstate
Chemicon’s Taq Polymerase Reagents were designed to let you Discover More & Publish Faster™. These reagents offer researchers choice recombinant thermostable DNA polymerases for real time PCR (qPCR). Because qPCR has become the method of choice to rapidly and quantitatively examine the expression of specific genes, there are two challenges in qPCR research; contaminating DNA, and degradation in mixes. Chemicon has Taq amplification kits optimized to prevent non-specific results and degradation. |
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Taq Hot Start DNA Polymerase
Takara Mirus Bio
Takara’s Hot-Start Taq DNA Polymerase contains a mixture of Takara’s high-purity Taq DNA Polymerase and a monoclonal antibody to Taq, which binds to the polymerase until the temperature is elevated. The binding of this antibody prevents nonspecific amplification due to mispriming and/or formation of primer dimers during reaction assembly. Taq HS Polymerase provides the specificity, efficiency, and reliability required for demanding applications such as multiplex PCR. |
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