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| RNA interference (RNAi) is widely used as a means of knocking down gene expression in order to characterize gene function. The trick is identifying the siRNA or miRNA that will successfully knockdown the function of your gene of interest in your cell type of interest. The vendors listed below have developed an array of tools to help out. Whether you want to generate your own construct for transfection or purchase a predesigned sequence, the vectors, probes and siRNAs listed below all come with guarantees for success. For detection of miRNA, check out the MicroRNA Assays, designed to quantify only mature miRNAs (not precursors). With the products listed below, you won’t have to fight for great results. |
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BLOCK-iTT Pol II miR RNAi Expression Vector Kits
Invitrogen
The BLOCK-iT™ Pol II miR RNAi Expression Vector Kits combine the advantages of traditional RNAi vectors - stable expression and the ability to use viral delivery - with capabilities for tissue-specific expression and multiple target knockdown from the same transcript. The pcDNA™6.2-GW/miR and pcDNA™6.2-GW/EmGFP-miR vectors included in the kits are designed to express artificial miRNAs which are engineered to have 100% homology to your target sequence and will result in target cleavage. |
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Plasmid-Based siRNA
Upstate
Mammalian cells express endogenous, short genes using RNA polymerase III enzymes and promoters. Upstate’s plasmid-based siRNA constructs utilize this endogenous system to express short-hairpin RNA (shRNA) from pKD™ plasmids. The shRNA is ultimately processed by the cell to form a functional siRNA . Upstate validates every plasmid in cell culture before release. Upstate guarantees knock-down of least 70% on the mRNA or protein level. The mRNA levels are determined using quantitative, real-time PCR, and protein levels via Western blots. |
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TaqManŽ MicroRNA Assays
Applied Biosystems
TaqMan® MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of gold standard TaqMan® Assay chemistry. Choose from hundreds of new, individual real-time PCR assays for human, mouse and rat. This new assay collection offers the ultimate flexibility in your miRNA research, with assays for C.elegans, Arabidopsis, and Drosophila coming soon. Only 1-10 nanograms of total RNA, or equivalent, are required to obtain high quality results in less than three hours. |
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Effective stable low toxicity microRNA knockdown with miRCURY LNA Knockdown Probes
Exiqon
Researchers can now determine miRNA function using knockdown probes based on the highly sensitive and specific nucleic acid analogue Locked Nucleic Acid (LNAT). LNAT has enabled in situ detection and array profiling of miRNAs with a degree of sensitivity and specificity that is not achievable with standard oligonucleotide chemistry. miRCURYT LNA Knockdown probes exploit the superior affinity of LNAT oligonucleotides to produce effective reduction of target miRNA levels and help determine which cellular processes are impacted upon by the miRNA |
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Guaranteed Silencing with Silencer® Pre-designed siRNAs
Ambion
Ambion's Silencer® Pre-designed siRNAs—available for human, mouse, and rat genes—are designed using an extensively tested algorithm and synthesized in Ambion's state-of-the-art facilities. The result is guaranteed gene silencing. Special pricing is now available when you purchase 3 siRNAs to the same target. |
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