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When it comes to PCR, we're all looking for the quickest way to perform specific and accurate reactions that generate high yields in a short amount of time. That may sound like too much to ask for, but the reagents listed below will help you achieve just that. Hot start PCR mixes increase specificity by preventing false-priming events which can occur during reaction set up. Modified Taq enzymes offer increased accuracy and processivity; a more processive enzyme generates more product in less time. To top it off, many of these options are available in master mixes so that all you have to do is add your primers, template and water and your reactions are ready to go. Whatever the goals of your PCR experiments, the products listed below will help you meet them quickly and efficiently.
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AccuPrimeT Taq DNA Polymerase and SuperMixes
Invitrogen
AccuPrime™ Taq DNA Polymerase and SuperMixes provide reagents for amplification of nucleic acid templates with antibody-mediated hot-start for improved PCR specificity over other hot-start DNA polymerases. Platinum® anti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. |
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Reagents For Quick And Efficient PCR
When it comes to PCR, we’re all looking for the quickest way to perform specific and accurate reactions that generate high yields in a short amount of time. That may sound like too much to ask for, but the reagents listed below will help you achieve just that. Hot start PCR mixes increase specificity by preventing false-priming events which can occur during reaction set up. Modified Taq enzymes offer increased accuracy and processivity; a more processive enzyme generates more product in less time. To top it off, many of these options are available in master mixes so that all you have to do is add your primers, template and water and your reactions are ready to go. Whatever the goals of your PCR experiments, the products listed below will help you meet them quickly and efficiently. |
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AccuPrimeT Taq DNA Polymerase and SuperMixes
Invitrogen
Invitrogen
AccuPrime™ Taq DNA Polymerase and SuperMixes provide reagents for amplification of nucleic acid templates with antibody-mediated hot-start for improved PCR specificity over other hot-start DNA polymerases. Platinum® anti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. |
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Ultra-High Fidelity, Pfu-Based Fusion DNA Polymerase
Stratagene
Our PfuUltra™ II Fusion HS DNA Polymerase sets a new standard in ultra-high fidelity PCR performance. Our PfuUltra II enzyme provides you the highest accuracy among all commercially available proofreading enzymes and shortens overall run time by 70-80%. The DNA polymerase has been fused to a DNA-binding protein to increase processivity by 12-fold over traditional PCR enzymes. Together with our exclusive ArchaeMaxx® Polymerase-Enhancing Factor and hotstart antibody, our PfuUltra II enzyme delivers you unmatched reliability, specificity, and target-length capability. |
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High Capacity cDNA Reverse Transcription kit high performance and inexpensive complete first strand synthesis kit from Applied Biosystems
The High Capacity cDNA Reverse Transcription kit is identical to the High Capacity cDNA Archive kit but with new configurations. The kits are configured to smaller sizes and with or without RNase Inhibitor. Linear conversions of total RNA over 7 logs are possible up to a 2ug in a 20uL reaction. Obtain enormous capacity to convert total RNA at a fraction of the competition’s cost. |
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HotStart-IT™ Taq DNA Polymerase
USB Corp
USB’s HotStart-IT™ Taq DNA Polymerase uses a novel hot start method called primer-sequestration. This new method does not use antibodies or chemically modified enzymes. A recombinant protein binds and sequesters primers at lower temperatures making them unavailable for use by a PCR enzyme. This technology blocks DNA synthesis from mis-priming events at lower temperatures. Following the initial denaturation step during PCR, the protein is inactivated and the primers are free to participate in the amplification reaction. This novel hot start method enhances many complex PCR reactions by increasing both specificity and yield. |
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Simplify hot start PCR with FastStart PCR Master
Roche Applied Science
Make high-performance hot start qualitative PCR virtually effortless with new FastStart PCR Master from Roche Applied Science. Simply add primers, template, and water to this convenient, time-saving mix to obtain high PCR sensitivity and specificity with FastStart Taq DNA Polymerase and PCR-Grade dNTPs. Easily prepare large numbers of reactions (e.g., with robotic pipetting instruments) with a heat-activated hot start mix that prevents non-specific amplification during set up. |
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Probes for Real-time qPCR Multiplexing
Biosearch Technologies
Success with multiplex qPCR demands efficient quenchers paired with high-performance fluorescent reporters having emission characteristics tuned to the optics of real-time thermocyclers. The Black Hole Quencher®, CAL Fluor and Quasar dyes from Biosearch Technologies were formulated precisely for optimum performance on today’s thermocyclers. Available for 5’, 3’ and internal-labeling of oligos, these dyes can be incorporated into all probe and primer designs, including TaqMan®, Molecular Beacon, Black Hole Scorpions™, Amplifluor® Direct and Plexor™ Primers. |
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