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Along with your protein of interest, cell lysis releases numerous proteases. Selectively and effectively inhibiting these proteases is an important part of any protein purification scheme. The range of protease inhibitors is as broad as the types of proteases they target. Preparing these inhibitors for use often means weighing and making solutions of numerous chemicals, such as PMSF and leupeptin. Commercially available protease inhibitor cocktails, however, have made inhibition of cellular proteases as easy as pipetting one solution or dropping a few tablets into your extraction buffer. The products listed below will effectively inhibit a variety of cellular proteases from a range of species. In addition to convenience, since these products are quality-control tested, you'll also find they perform more consistently than most homemade cocktails. So peruse the products below and rest assured that you're on your way to more effective, convenient and consistent inhibition, and thus, cleaner, clearer results in all of your downstream applications.
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ProteCEASET Protease Inhibitor Cocktails
G Biosciences
ProteCEASE™ protease inhibitor cocktails contain optimized concentrations of various protease inhibitors, which provide superior inhibition of protease activities during protein purification from a wide selection of species. ProteCEASE™ has been specifically developed for large scale preparative applications and is available in two sizes for 50ml or 100ml of lysis buffer. ProteCEASE™ is available with EDTA, to inhibit metalloproteases, or without EDTA, to maintain activity of proteins dependent on divalent cations. The EDTA-free ProteCEASE™ will not inhibit the purification of proteins with immobilized metal affinity chromatography. The convenient format ensures the delivery of consistent concentrations of protease inhibitors that have high protease inhibition (>90% in mouse pancreas lysate). |
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AEBSF hydrochloride BioChemica
AppliChem GmbH
AEBSF irreversibly inactivates thrombin and other serine proteases (e. g. chymotrypsin, kallikrein, plasmin, proteinase K, trypsin) by sulfonylation of a functional group in the active center of the enzyme. AEBSF is a substantially less toxic substitute for PMSF and DFP. It is highly water-soluble and its solutions have an acidic pH. At this pH, stability is high |
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Complete Protease Inhibitor Cocktail Tablets
Roche Applied Science
Used for the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts in large volumes. The tablets are easy to use; simply drop a quick-dissolving tablet into your buffer. They offer complete protein protection against a broad range of proteases. This cocktail performs in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi. With the Protease Inhibitor Cocktail Tablets, Roche supplies non-toxic inhibitors without risk to you or those around you. |
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