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The longer the amplicon, the greater the challenge for standard Taq polymerase. Fortunately, there is a selection of enzymes capable of amplifying 10, 20 even as many as 40+ kilobases of DNA. Some of these enzymes are modified forms of Taq; others are proprietary mixes of different versions of Taq, optimized to amplify long stretches of DNA. In addition to performing long PCR, many of these enzymes also possess proofreading capabilities, so your PCR product is ready for a myriad of applications. You can also choose to purchase pre-mixed buffer solutions to further simply your reaction optimization and set-up. Check out the products listed below to amplify all of your long targets
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iProof™ High-Fidelity DNA Polymerase
Bio-Rad
iProof high-fidelity polymerase comprises a unique proofreading enzyme fused to a dsDNA binding protein, Sso7d. This novel technology produces a thermostable polymerase, capable of fast PCR, with the highest fidelity available (52-fold more accurate than Taq). iProof polymerase is available in three convenient formats: a stand-alone enzyme, an easy-to-use master mix, and a PCR kit that includes controls. |
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MasterAmp™ Extra-Long PCR Kit
EPICENTRE Biotechnologies
The MasterAmp™ Extra-Long PCR Kit enables successful and accurate one-step amplification of DNA sequences from 20 kb to 40 kb in an easy to use optimization format. For each new set of template and primers simply perform a PCR reaction with the MasterAmp Extra-Long DNA Polymerase Mix and the 9 PreMixes, all conveniently included in each kit, to find your optimal PreMix. At least one PreMix should amplify your long template and give consistent results in all subsequent amplifications |
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Expand Long Range dNTPack
Roche Applied Science
Obtain the Long PCR Products Your Research Requires with the Expand Long Range dNTPack Choose Expand Long Range dNTPack as your complete solution for the consistent amplification of PCR products of 5 to 25 kb from genomic DNA. This product combines optimized buffers, ultrapure PCR-grade nucleotides, and a next-generation blend of thermostable enzymes in a single convenient kit. Take advantage of the enzymes’ high fidelity to perform a variety of applications, including genome mapping, sequencing, contig construction, and cloning and analysis of eukaryotic genes. |
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